Given the infinite quantity of compounds and structural variety of probable analytes, HPLC is never a a person-dimensions-suits-all method. From nano to preparative scale separations, Here's a listing of the commonest varieties of HPLC techniques and when to use Every.Separation sciences have contributed immensely to The expansion and comprehension

Hydroxyl worth: the mass in milligrams of KOH akin to hydroxyl groups in one gram of sample. The analyte is acetylated utilizing acetic anhydride then titrated with KOH.A titration is often a volumetric technique wherein a solution of 1 reactant (the titrant) is included to a solution of a second reactant (the "analyte") right until the equivalence